Biology Biotechnology Principles and Processes Tools Of Recombinant DNA Technology Single Correct MCQ
Published on: August 14, 2026

All of the following are methods of insertion of recombinant DNA into host cells except-

A
Incubation of bacterial cells with recombinant DNA on ice followed by heat shock and transferring to ice
B
Use of disarmed pathogen vectors
C
PCR
D
Biolistics

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Text Solution

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The correct answer is:
C

Heat shock method → Yes, used to introduce plasmid DNA into bacteria

Disarmed pathogen vectors → Yes, used in plant transformation

PCR → Incorrect, because PCR is used for amplification of DNA in vitro, not for inserting DNA into host cells

Biolistics (gene gun) → Yes, used to deliver DNA into plant cells.

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